The aim of this study was to evaluate the antibacterial activity of 28 plant extracts and oils. In vitro antibacterial activity of several plant extracts. Ten plant extracts were analysed for their antimicrobial activities. Antimicrobial activity of selected medicinal plants. External infections involving the skin and wound are the most frequent complications affecting humans and animals. Frontiers antimicrobial properties and mechanism of. Egamberdieva d, wirth s, behrendt u, ahmad p and berg g 2017 antimicrobial activity of medicinal plants correlates with the proportion of antagonistic endophytes. Azadirachta indica leaves and barks were collected from. Antibacterial and antifungal activities from leaf extracts. Antimicrobial activity in leaf extract of neem azadirachta indica against human pathogenic bacteria. Medicinal plants play great roles in the treatment of skin and wound infections. Nicolson is a tropical medicinal palnt belonging to rutaceae family. The results revealed that all plant extracts were potentially effective in suppressing microbial growth of food poisoning bacteria with variable potency. The aim of this study was to evaluate the antibacterial activity of 28 plant extracts and oils against four gramnegative bacterial species.
Antimicrobial resistance occurs when microorganisms which were initially sensitive to antimicrobial drugs becomes ineffective when exposed to them by not killing. Its subdisciplines are virology study of viruses, bacteriology study of bacteria. The plants were selected on the basis of their reported. The results obtained with pseudomonas aeruginosa was particularly interesting, since it was inhibited by clove, jambolan, pomegranate and thyme extracts. Antibacterial potency of combined plant extracts has been tested against bacillus subtilis ifo 3026, sarcina lutea ifo 3232, xanthomonas campestris iam 1671, escherichia coli ifo 3007, klebsiella pneumoniae attc 10031, proteus vulgaris mtcc 321 and pseudomonas denitrificans kacc 32026 by disc diffusion assay. The antimicrobial effects of the plant extracts are sufficient in a way to cater the healing effect. The antibacterial activity of extracts of nine plant. Commercially available standard antibiotic discs were also used to find out. In vitro antimicrobial activity of some medicinal plants. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. The antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts ishaku leo elisha 1. Acute diarrhea, medicinal plants, antibacterial, resistance, minimum inhibitory concentration. Antimicrobial activity of medicinal plants correlates with.
Antibacterial activity of seed and leaf extract of carica papaya var. Pdf antimicrobial activity of medicinal plant leaf. Varying concentration of each extracts 200mgml, 150 mgml, 100mgml. Preliminary studies have been conducted into the antibacterial activity of extracts from some local medicinal plants from the family connaraceae. The aim of the study was to evaluate the antifungal activity of extracts of 10 plant species used in traditional uruguayan medicine against the phytopathogenic fungus alternaria spp. Antibacterial activity of plant extracts against selected. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. In vitro alireza massiha,1 mohammadreza majid khoshkholghpahlaviani, 2 khosro issazadeh, 1 sirus bidarigh, 3 saeed zarrabi4 1.
Biochemistry graduates to have results from their final year research projects on the antibacterial activity of plant extracts submitted for publication dilruba meah and aiysha thompson have just finished their biochemistry. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibioticresistant bacteria. Aside from the crude extract from the rotary evaporator, three fractions from the plant were prepared using methanol, dichloromethane dcm and hexane. The antibacterial activity of ethanol extract of piper nigrum showed against escherichia coli, salmonella typhi.
Green synthesis of silver nanoparticles in aloe vera plant. The extracts exhibited antibacterial activity at concentrations of 25 and 10 mgml with mbc of both extracts ranged between 3. Pdf antibacterial and antifungal activity of plant extracts. Antibacterial and antifungal activity of plant extracts 221 synthesis of the obtained results t able 6 showed that the activi ty of the extracts depended on the sp ecies of plants, a method of. Collection of plant material and extract preparation. Antimicrobial activity antibacterial activity introduction the science dealing with the study of the prevention and treatment of diseases caused by microorganisms is known as medical microbiology.
The highest total phenolic content tpc was detected in c. Antimicrobial assay the antimicrobial activity of the plant extracts was evaluated using agar well diffusion method 21 with minor modifications. Phytochemical analysis and antibacterial activity of pepper piper nigrum l. A 1 ml of an overnight culture of each bacterial isolate equivalent to 107810 cfu ml1 was used to seed sensitivity test. As possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease. The antibacterial effect of some medicinal plant extracts. Plant extracts showed in vitro antibacterial activity. It is in view of this, that the present research was set up to evaluate the antimicrobial activity of, using different. Screening of indian plant extracts for antibacterial activity. Pdf antibacterial activity of medicinal plant extracts. The antibacterial activity of leaf extracts of eucalyptus. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, staphylococcus aureus atcc 25923 and escherichia coli atcc 25922, by.
Evaluation of antibacterial activity of medicinal plant. Antimicrobial activities of alcoholic extracts of neem leaves were used. The antibacterial activity of the extracts was determined using the agar cup diffusion as described by adeniyi et al 1. Antimicrobial activity of some plant extracts against.
Plant volatile oils are generally isolated from nonwoody plant material by distillation methods, usually steam or hydrodistillation, and are. To enhance our understanding of antimicrobial activity mechanism of plant extracts, the changes in internal ph phin, and membrane potential were measured in staphylococcus aureus sa, and escherichia coli ec cells after exposure to the plant extracts. Antibacterial activity of combined medicinal plants. Evaluation of antimicrobial activity of different solvent. In this research, the antimicrobial activities of chloroformic and methanolic extracts of sophora flavescens, rhaponticum repens, alhagi maurorum, melia azedarach, peganum harmala, and. Materials and methods plates were prepared for the antibacterial activity. Antimicrobial activity of selected medicinal plants against some selected. The plant extracts showed antibacterial activity but not antifungal activity against any of the fungi used. Pdf antibacterial activity of the plant extract of. Antibacterial australian plants griffith university. The aim of the study was assess the antibacterial effect of some medicinal plant extracts and their synergistic antibiotic and nonantibiotic drugs against escherichia coli, staphylococcus aureus and pseudomonas aeruginosa.
Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the requirements for the degree of master of science. Plant extracts sriyeta chakraborty1, souvagyalaxmi sahoo1, anjana bhagat2, sangita dixit2. To avoid the chemical toxicity, biosynthesis green synthesis of metal nanoparticles is proposed as a cost. Antibacterial activity of leaves crude extracts of psidium guajava on pathogenic bacteria sr. The antiseptic qualities of aromatic and medicinal plants and their extracts have been recognized since antiquity, while attempts to characterize these properties in the laboratory date back to the early 1900s martindale 1910. Each purified extracts were dissolved in dimethyl sulfoxide, sterilized by filtration using sintered glass filter, and stored at 4c. Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens desalegn amenu college of natural and computational science, biology department, wollega university, p. In vitro antibacterial activity of selected medicinal. Antibacterial activity of seed and leaf extract of carica. The antimicrobial effect of spices extracts also helps to prevent diseases in many forms. Antimicrobial activity of the extracts was determined by disc diffusion assays. The results of antibacterial activity of the extracts and their potency were comparatively assessed by the diameter of zone of inhibition in millimeters, excluding the well diameter table 1. Antibacterial activity of the plant extract of symphytum officinale l.
Significant inhibitory activity was observed with terpenoid extract of the plant against the test bacteria while less antibacterial activity was observed in, flavonoid and glycoside extracts. Indeed, of the twenty five plant species tested only 3 species had no inhibitory activity towards any of the bacteria tested in any of their. Total extractives of four of the five plants examined showed activity against staphylococcus aureus, bacillus subtilis, escherichia coli and pseudomonas aeruginosa. The purpose of this study was to investigate the composition of leaf extracts from aronia melanocarpa, chaenomeles superba, and cornus mas, and their antimicrobial activity against typical spoilagecausing and pathogenic bacteria found in meat and meat products.
Twenty nine of the thirty nine extracts tested 72% showed antibacterial activity against one or more bacteria. Biochemistry graduates to have results from their final. Secondly, the method used to assess antimicrobial activity, and the choice of test organisms, varies between publications janssen et al. Although some extracts exhibited a good antibacterial activity towards different tested bacterial isolates, many plant extracts exhibited a limited antibacterial activity against the test bacterial isolates as judged by their mic values. Evaluation of antimicrobial activity of some plant. The 4 solvent extracts were then evaluated for antimicrobial activity using disc diffusion method on 18 strains of organisms.
Antibacterial activity of essential oils and plant extracts of artemisia artemisia annua l. Phytochemical analysis and antibacterial activity of. Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in the treatment of antibioticresistant bacteria. Most plant extracts exhibited inhibitory activity against one or more tested bacterial strains, but plant extracts displaying. Antimicrobial activity of essential oils and other plant. Hypericum perforatum, ziziphora capitata, endophytic bacteria, plant growth traits, antimicrobial activity, antagonism. The screening of plant extracts has been of great interest to scientist for the discovery of new drugs. Assay for antimicrobial activity antimicrobials are agents that kill microorganisms or inhibit their growth. A method frequently used to screen plant extracts for antimicrobial activity is the agar disc diffusion technique morris et al. Antimicrobial activity of crude extracts of some plant leaves. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig. All the plant extracts were screened for their antimicrobial activity.
Moreover, antimicrobial activity of different natural substances such as medicinal plant extract have been investigated against food borne bacteria. A study on the antioxidant and antimicrobial activities in. Background there is worldwide interest in silver nanoparticles agnps synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. The investigation of antimicrobial activity as well as cell toxicity of extracts from 30 plant species against five bacteria species and two fungi species was studied 29.
Water and methanol extracts of both plants represented good activity against most of the sensitive and. Box, 395, ethiopia abstract antibiotics provide the main basis for the therapy of microbial bacterial and fungal infections. It was concluded that ethanol extracts from 70 % of the plants were toxic to cell and only one of the species of combretum duarteanum showed antimicrobial activity. Antibacterial activity of essential oils and plant. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. Medicinal plants, ethanol, methanol and aqueous extracts, human pathogens, antimicrobial activity. Minimum inhibitory concentration mic assay were determined for these two extracts. Antimicrobial activity of medicinal plant extracts and.